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Download Animal Cell Technology. Developments, Processes and Products by R. E. Spier PDF

By R. E. Spier

This e-book offers a cutting-edge file at the box of animal cellphone expertise, a resource of reference for all these occupied with the construction and use of animal cellphone items

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Glutamine- free serum- free fed batch processes for both CHO and gs- NSO cell lines have been developed which meet these criteria. Performance of high yielding gs-CHO and gs-NSO cell lines producing recombinant antibodies is described. RESULTS AND DISCUSSION I. Stability of gs-CHO Cell Lines Stability of gs-CHO cell lines was assessed by studying three different cell lines. 3 recognising the TAG72 tumour marker (3). The third gs-CHO cell line produced a recombinant antibody recognising a different antigen (rmAB-3).

In the leukocytes immortalised by cotransfection of pRcCMV-c-mjc with pRcCMV-c-Ha-ras, the ratio of lymphocytes was remarkably increased compared with that of lymphocytes in primary cultured cells (Table 1). DISCUSSION myc and ras are effective on transformation of mammalian cells(3). The signal of ras is thought to be transfered to nucleus with the induction of fos(3). Thus the combination of fos and ras might be more effective on immortalisation of flatfish leukocytes than unique fos. Flatfish leukocyte cell lines were easily produced with oncogene transfection.

No difference was found in the number of colonies formed under GHT-selection. 4 times more colonies at the 1 μΜ Mtx level than did the control. 7 times more colonies than the control at the two Mtx levels (data not shown). 3) CD4-lgG expression levels in co-transfections with A-type retroviral sequences. 4 kb A-type subclone were used. These DHFR plasmids were co-transfected at a 1:10 molar ratio with an expression vector for CD4-lgG. Cells from randomly chosen colonies were cloned, expanded to individual cell lines and assayed for their expression of CD4-lgG.

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